For example, a horizontal line can be added to show the difference between multiple datasets. Select the option that suits your intention. The lines with text option can be found at the bottom of the draw tool’s dropdown menu. There are a plethora of options available however, the ones that concern us are the ‘ lines with text‘ options. With the graph open in Prism, click on the draw tool at the top. How to add significance lines and symbols to graphs in PrismĪdding significance lines and symbols is incredibly easy in Prism! Simply follow the steps below. The significance line and symbol can be used to show that the two datasets are significantly different from one another. These lines usually have a symbol (eg *) or text (eg P<0.05) to provide additional information to the reader. I’m talking about the horizontal, and sometimes zig-zag, lines that are added onto graphs to indicate when significant differences are present between datasets. I don’t believe the correct term is significance lines but I am going to use this hereafter. In this tutorial I will explain how to use GraphPad Prism to add lines with text to depict statistical significance between datasets on graphs.

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